FIG 2.

HIV sequence copies per million cells using ddPCR with DNA from PBMCs from 9 subjects not receiving ART, before or after PFGE. HIV gag was measured to capture both proviral DNA and HIV episomes. 2-LTR primers were used to measure 2-LTR circular episomes. (a) HIV gag and 2-LTR circle copy numbers for the individual subjects. (b) Median values for the 9 subjects. The median gag copy number before PFGE was 2,227 copies per million cells (95% CI, 1,006 to 2,769 copies per million cells). The median gag copy number after PFGE was 542 copies per million cells (95% CI, 210 to 1,584 copies per million cells) (P = 0.0078). The median 2-LTR circle copy number before PFGE was 22 copies per million cells (95% CI, 0.76 to 120 copies per million cells); after PFGE, no 2-LTR circles were detected in any of the samples (P = 0.0078). The median recovery of genomic DNA of 37% (95% CI, 28.9 to 42.8%) was determined by measuring the host cell RPP30 gene.