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. 2018 Nov 27;56(12):e01329-18. doi: 10.1128/JCM.01329-18

FIG 2.

FIG 2

Ion-exchange chromatography (IEX) of the insoluble fraction of B. henselae Houston-1 and line blot analysis of the respective fractions by using human reactive sera. (A) Chromatogram of the IEX with the PBS-insoluble antigens from B. henselae Houston-1. Shown are the UV signal (solid line) and the gradient for elution (dotted line). Arrows indicate fraction 11 and fraction 24. (B) Line blots with fractions collected after the IEX. Blots were incubated either with IFA-positive patient sera (n = 7, left) or with IFA-negative sera (n = 6, right). The row with the best ratio (fraction 11) and with the best difference (fraction 24) are marked with arrows. (C) Visualization of the reactivity of the line blots with human sera by heat maps. Values are shown ranging from no binding (green) to maximum binding (red). Numbers at the side indicate the number of the particular IEX fraction (ce, crude extract).