Figure 2.

hAAT Inhibited BM pDC Maturation and Cytokine Secretion

BM pDCs from B6.TC mice were differentiated in vitro using Flt3l with or without hAAT for 8 days and then stimulated or not with 10 μg/mL CpG for an additional 24 hr prior to FACS analysis. (A) Representative FACS plots showing the MFI of costimulatory molecules CD40 and CD80 and the frequency of PDCA-1⁺ CCR9⁺ cells, gated on PDCA-1^hi CD11c^int. (B) Statistical analysis for CD40 and CD80 expression and frequency of the CCR9⁺ PDCA-1⁺ in pDCs. (C) IL-6 and TNF-α levels in supernatants. Data were analyzed by one-way ANOVA using Tukey’s post hoc test corrections. n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001.