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. 2018 Oct 23;11:155–165. doi: 10.1016/j.omtm.2018.10.008

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Affinity Chromatography Purification of cTag8 LVs Using Streptavidin Monolith Column

(A) Diagram of the CIMac streptavidin (0.1-mL) analytical column purification step process, with indicated collected fractions (5 × 10 mL flow-through fractions; 2 × 15 mL wash fractions; 6 × 1.5 mL [1 to 6], and 1 × 7.5 mL [7] eluate fractions), which were assessed for their viral titers by infectivity assay. (B) Viral titers of collected fractions, and (C) the yield of each fraction in terms of total transducing units (TU), which are represented as a percentage of starting input transducing units. Values are presented ± SD of triplicate determinations for each fraction.