Table 1.
Strain or plasmid | Relevant genotype or characteristic(s) | Reference or source |
---|---|---|
STRAIN | ||
MC4100 | F- araD139 (argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150 | Casadaban, 1976 |
RM220 | As MC4100, but ΔpflAB | Kaiser and Sawers, 1994 |
FTD147 | As MC4100, but ΔhyaBΔhybCΔhycE | Redwood et al., 2008 |
FTD147ΔfdnG | As aFTD147, but ΔfdnG | This work |
FTD147ΔfdoG | As aFTD147, but ΔfdoG | This work |
FTD147ΔfdhF | As aFTD147, but ΔfdhF | This work |
FTD147ΔfdnGΔfdoG | As aFTD147, but ΔfdnG ΔfdoG | This work |
FTD147ΔfdnGΔfdhF | As aFTD147, but ΔfdnG ΔfdhF | This work |
FTD147ΔfdoGΔfdhF | As aFTD147, but ΔfdoG ΔfdhF | This work |
FTD147ΔfdnGΔfdoGΔfdhF | As aFTD147, but ΔfdnG ΔfdoG ΔfdhF | This work |
FTD147 ΔselC | As FTD147, but ΔselC KanR | This work |
FTD150 | As MC4100, but ΔhyaBΔhybCΔhycE ΔhyfG | Redwood et al., 2008 |
FTD150ΔselB | As FTD150, but ΔselB KanR | This work |
CP1170 | As MC4100, but ΔhyaBΔhybCΔhycA-I | This work |
CPH008 | As MC4100, but ΔhycA-I ΔfdhF | This work |
CPH020 | As MC4100, but ΔhyaBΔhybCΔhycA-IΔfdhF | Pinske, 2018 |
CPH021 | As MC4100, but ΔhyaBΔhybCΔhycA-IΔfdhE | This work |
RT2 | As MC4100, but ΔhyaBΔhybCΔhycA-IΔfdhEΔpflA | Pinske and Sargent, 2016 |
PLASMIDS | ||
pCP20 | FLP+, λcI857+, λpR Repts, AmpR, CmR | Cherepanov and Wackernagel, 1995 |
bpSHH18 | pACYC-Duet-I, hupXSLhoxM+ CmR = phupXSL | Hartwig et al., 2015b |
phupSL | pACYC-Duet-I, hupSLhoxM+, CmR | This work |
phupX | pACYC-Duet-I, hupX+, CmR | This work |
aThe series of FTD147 mutants with different combinations of Fdh gene mutations was constructed by transduction of mutations from the corresponding Keio collection of mutants (see Materials and Methods). bNote that for reasons of clarity, this plasmid was referred to as phupXSL throughout this study.