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. 2018 Nov 21;9:2837. doi: 10.3389/fmicb.2018.02837

Table 1.

Strains and plasmids used in this study.

Strain or plasmid Relevant genotype or characteristic(s) Reference or source
STRAIN
MC4100 F- araD139 (argF-lac)U169 ptsF25 deoC1 relA1 flbB5301 rspL150 Casadaban, 1976
RM220 As MC4100, but ΔpflAB Kaiser and Sawers, 1994
FTD147 As MC4100, but ΔhyaBΔhybCΔhycE Redwood et al., 2008
FTD147ΔfdnG As aFTD147, but ΔfdnG This work
FTD147ΔfdoG As aFTD147, but ΔfdoG This work
FTD147ΔfdhF As aFTD147, but ΔfdhF This work
FTD147ΔfdnGΔfdoG As aFTD147, but ΔfdnG ΔfdoG This work
FTD147ΔfdnGΔfdhF As aFTD147, but ΔfdnG ΔfdhF This work
FTD147ΔfdoGΔfdhF As aFTD147, but ΔfdoG ΔfdhF This work
FTD147ΔfdnGΔfdoGΔfdhF As aFTD147, but ΔfdnG ΔfdoG ΔfdhF This work
FTD147 ΔselC As FTD147, but ΔselC KanR This work
FTD150 As MC4100, but ΔhyaBΔhybCΔhycE ΔhyfG Redwood et al., 2008
FTD150ΔselB As FTD150, but ΔselB KanR This work
CP1170 As MC4100, but ΔhyaBΔhybCΔhycA-I This work
CPH008 As MC4100, but ΔhycA-I ΔfdhF This work
CPH020 As MC4100, but ΔhyaBΔhybCΔhycA-IΔfdhF Pinske, 2018
CPH021 As MC4100, but ΔhyaBΔhybCΔhycA-IΔfdhE This work
RT2 As MC4100, but ΔhyaBΔhybCΔhycA-IΔfdhEΔpflA Pinske and Sargent, 2016
PLASMIDS
pCP20 FLP+, λcI857+, λpR Repts, AmpR, CmR Cherepanov and Wackernagel, 1995
bpSHH18 pACYC-Duet-I, hupXSLhoxM+ CmR = phupXSL Hartwig et al., 2015b
phupSL pACYC-Duet-I, hupSLhoxM+, CmR This work
phupX pACYC-Duet-I, hupX+, CmR This work

aThe series of FTD147 mutants with different combinations of Fdh gene mutations was constructed by transduction of mutations from the corresponding Keio collection of mutants (see Materials and Methods). bNote that for reasons of clarity, this plasmid was referred to as phupXSL throughout this study.