Table 2.
H2:BV oxidoreductase activities of E. coli strains carrying phupXSL.
| Strain | Anaerobic hydrogenase activity (mU mg protein-1) | Activity after O2 exposure (mU mg protein-1)a |
|---|---|---|
| CP1170 (ΔhyaB,ΔhybC,ΔhycA-I) | 10 ± 3b | n.dc |
| FTD147 (ΔhyaB,ΔhybC,ΔhycE) + phupXSL | 58 ± 18 | 29 ± 9 |
| FTD147ΔfdnG,ΔfdoG | 26 ± 13 | n.d. |
| FTD147ΔfdnG,ΔfdoG + phupXSL | 59 ± 1 | 22 ± 1 |
| FTD147ΔfdhF | 4 ± 2 | n.d. |
| FTD147ΔfdhF + phupXSL | 11 ± 1 | n.d |
aCrude extracts were exposed to air for 15 min prior to determination of hydrogenase activity. bAssays were performed in triplicate from 3 independent growth experiments. The activity is shown together with the standard deviation. cn.d, not determined.