REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Escherichia coli, OP50	Caenorhabditis Genetics Center	WormBase ID: OP50
Escherichia coli, DH5α	ThermoFisher Scientific	Cat#8265017
Escherichia coli, DH5β	NEB	Cat#C30191
Chemicals, Peptides, and Recombinant Proteins
Aldicarb	Sigma	Cat#33386
Sytox green	Sigma	Cat#S7020
Latrunculin A	Sigma	Cat#L5163
Critical Commercial Assays
cDNA synthesis kit	Roche	Cat#04897030001
SYBR Premix Ex TaqII master mix	Clontech	Cat#RR820B
RNeasy Plus Mini Kit	QIAGEN	Cat#74136
Experimental Models: Organisms/Strains
N2 C.elegans wild isolate	Caenorhabditis Genetics Center	WormBase ID: N2
Mutant hic-1(ok3475) X	Caenorhabditis Genetics Center	Strain RB2512
Mutant nab-1(ok943) I	Caenorhabditis Genetics Center	Strain RB1017
Mutant acr-16(ok789) V	Caenorhabditis Genetics Center	Strain RB918
Mutant mig-14(mu71) II	Caenorhabditis Genetics Center	Strain CF367
Mutant lin-17(n671) I	Caenorhabditis Genetics Center	Strain MT1306
Integrated line Punc-17::RFP	Josh Kaplan Lab (Babu et al., 2011)	nuIs321
Integrated line Punc-25::GFP	Caenorhabditis Genetics Center	juIs76 CGC strain number CZ1200
Integrated line Punc-129::GFP::SNB-1	Josh Kaplan Lab (Sieburth et al., 2005)	nuIs152
Integrated line Punc-25::SNB-1::GFP	Josh Kaplan Lab (Hao et al., 2012)	nuIs376
Integrated line Pacr-2::mCherry::RAB-3	Mike Francis Lab (Petrash et al., 2013)	uIfs63 strain pPRB47
Integrated line Punc-129::SYD-2::YFP	Josh Kaplan Lab (Sieburth et al., 2005)	nuIs159
Integrated line Punc-25::SYD-2::GFP	Caenorhabditis Genetics Center (Yeh et al., 2005)	hpIs3 CGC strain number ZM54
Integrated line Pmyo-3::UNC-29::GFP	Villu Maricq lab (Francis et al., 2005)	akIs38
Integrated line Pmyo-3::ACR-16::GFP	Josh Kaplan Lab (Babu et al., 2011)	nuIs299
Integrated line Pmyo-3::UNC-49::GFP	Josh Kaplan Lab (Babu et al., 2011)	nuIs283
Integrated line Pmyo-3::GCaMP3.35	Caenorhabditis Genetics Center (Schwarz et al., 2012)	goels3 CGC strain number HBR4
Integrated line Pnab-1::NAB-1::GFP	Mei Zhen Lab (Hung et al., 2007)	hpIs66
Integrated line Punc-17:: GFP-UtrCH	This study	indIs001
Integrated line Punc-129::LIN-44::mCherry	This study, array number IndEx32 from (Pandey et al., 2017)	indIs002
Integrated line Punc-17::CWN-2::mCherry	This study	indIs003
Integrated line Punc-17::NLP-21::YFP	Derek Sieburth Lab (Staab et al., 2013)	vjIs30
Integrated line Punc-129::GSLN-1::YFP	Josh Kaplan Lab (Sieburth et al., 2005)	nuIs169
Plasmid BAB#0101 Phic-1::mCherry (injected into WT)	This study	Array IndEx001/strain BAB047
Plasmid BAB#0102 Phic-1::HIC-1::mCherry (injected into WT)	This study	Array IndEx002/strain BAB348
Plasmid BAB#0103 Prab-3::HIC-1 (injected into hic-1)	This study	Array IndEx003/strain BAB349
Plasmid BAB#0104 Plet-413::HIC-1 (injected into hic-1)	This study	Array IndEx004/strain BAB350
Plasmid BAB#0105 Pmyo-3::HIC-1 (injected into hic-1)	This study	Array IndEx005/strain BAB351
Plasmid BAB#0106 Punc-17::HIC-1 (injected into hic-1)	This study	Array IndEx006/strain BAB352
Plasmid BAB#0107 Punc-25::HIC-1 (injected into hic-1)	This study	Array IndEx007/strain BAB353
Plasmid BAB#0108 Punc-17::HIC-1 ΔC(4aa) (injected into hic-1)	This study	Array IndEx008/strain BAB354
Plasmid BAB#0110 Punc-17::NAB-1::VN173 (injected into uIfs63)	This study	Array IndEx009/ strain BAB355
Plasmid BAB#0111 Punc-17::HIC-1::VC155 (injected into uIfs63)	This study	Array IndEx010/ strain BAB356
Plasmid BAB#0112 Punc-17::HIC-1ΔC(4aa)::VC155 (injected into uIfs63)	This study	Array IndEx011/ strain BAB357
Plasmid BAB#0115 Phic-1::HIC-1ΔC(4aa)::mCherry (injected into nuIs152)	This study	Array IndEx012/ strain BAB358
Plasmid BAB#0114 Punc-25:: GFP-UtrCH (injected into WT)	This study	Array IndEx013/ strain BAB359
Plasmid BAB#0110 Punc-17::NAB-1::VN173 (injected into nab-1)	This study	Array IndEx014/ strain BAB360
Plasmid BAB#0109 Punc-17::CWN-2::mCherry (injected into WT)	This study	Array IndEx015/ strain BAB361
Plasmid BAB#0110, BAB#0111 Punc-17::HIC-1:: VC155;Punc17::NAB-1::VN173 (injected into uIfs63)	This study	Array IndEx016/ strain BAB362
Plasmid BAB#0110, BAB#0112 Punc-17::HIC-1ΔC(4aa):: VC155; Punc17::NAB-1::VN173 (injected into uIfs63)	This study	Array IndEx017/ strain BAB363
Plasmid BAB#0116 Punc-17:: HIC-1(ΔC(4aa)+NAB-1 (ABD)) (injected into BAB323)	This study	Array IndEx018/ strain BAB364
Plasmid BAB#0116 Punc-17:: HIC-1(ΔC(4aa)+NAB-1 (ABD)) (injected into BAB330)	This study	Array IndEx019/ strain BAB365
Plasmid BAB#0116 Punc-17:: HIC-1(ΔC(4aa)+NAB-1 (ABD)) (injected into BAB333)	This study	Array IndEx020/ strain BAB366
Plasmid BAB#0108 Punc-17::HIC-1 ΔC(4aa) (injected into BAB323)	This study	Array IndEx021/ strain BAB367
Plasmid BAB#0108 Punc-17::HIC-1ΔC(4aa) (injected into BAB330)	This study	Array IndEx022/ strain BAB368
Plasmid BAB#0108 Punc-17::HIC-1 ΔC(4aa) (injected into BAB333)	This study	Array IndEx023/ strain BAB369
Plasmid pCFJ68 Punc-122::GFP (injected into indIs003)	This study	Array IndEx024/ strain BAB370
Plasmid pCFJ68 Punc-122::GFP (injected into indIs002)	This study	Array IndEx025/ strain BAB371
hic-1(ok3475); nuIs321	This study	BAB302
hic-1(ok3475); juIs76	This study	BAB303
hic-1(ok3475); nuIs152	This study	BAB304
hic-1(ok3475); juIs376	This study	BAB305
hic-1(ok3475); uIfs63	This study	BAB306
hic-1(ok3475); nuIs159	This study	BAB307
hic-1(ok3475); hpIs3	This paper	BAB308
hic-1(ok3475); nuIs169	This paper	BAB309
hic-1(ok3475); nuIs169; IndEx006	This study	BAB310
hic-1(ok3475); akIs38	This study	BAB311
hic-1(ok3475); nuIs299	This study	BAB312
hic-1(ok3475); nuIs299; IndEx006	This study	BAB313
hic-1(ok3475); nuIs283	This study	BAB314
hic-1(ok3475); goels3	This study	BAB315
hic-1(ok3475); goels3; IndEx006	This study	BAB316
hic-1(ok3475); indIs001	This study	BAB317
hic-1(ok3475); indIs001; IndEx006	This study	BAB318
hic-1(ok3475); indIs002	This study	BAB319
hic-1(ok3475); indIs002; IndEx006	This study	BAB320
hic-1(ok3475); IndIs003	This study	BAB321
hic-1(ok3475); IndIs003; IndEx006	This study	BAB322
nab-1(ok943); hic-1(ok3475)	This study	BAB323
mig-14(mu71); hic-1(ok3475)	This study	BAB324
lin-17(sy277); hic-1(ok3475)	This study	BAB325
nab-1(ok943); IndEx014	This study	BAB326
nab-1(ok943); hpIs66	This study	BAB327
nab-1(ok943); IndIs001	This study	BAB328
nab-1(ok943); IndEx014; IndIs001	This study	BAB329
nab-1(ok943); hic-1(ok3475); IndIs001	This study	BAB330
nab-1(ok943); IndIs003	This study	BAB331
nab-1(ok943); IndEx014; IndIs003	This study	BAB332
nab-1(ok943); hic-1(ok3475); IndIs003	This study	BAB333
mig-14(mu71); IndIs003	This study	BAB334
mig-14(mu71); hic-1(ok3475); IndIs003	This study	BAB335
hic-1(ok3475); hpIs66	This study	BAB336
hpIs66; IndEx002	This study	BAB337
hic-1(ok3475); vjIs30	This study	BAB348
nab-1(ok943); nuIs299	This study	BAB349
nab-1(ok943); nuIs299; IndEx014	This study	BAB350
nab-1(ok943); hic-1(ok3475); nuIs299	This study	BAB351
hic-1(ok3475); acr-16(ok789)	This study	BAB352
hic-1(ok3475); hpIs66; IndEx006	This study	BAB355
hic-1(ok3475); IndEx024	This study	BAB356
hic-1(ok3475); IndEx006; IndEx024	This study	BAB357
mig-14(mu71); IndEx024	This study	BAB358
mig-14(mu71); hic-1(ok3475); IndEx024	This study	BAB359
hic-1(ok3475); IndEx025	This study	BAB360
hic-1(ok3475); IndEx006; IndEx025	This study	BAB361
Oligonucleotides
See Table S1 for primer sequences and information		N/A
Recombinant DNA
pPD49.26	Addgene	Plasmid#1686 (Andrew Fire lab)
pCFJ910	Addgene	Plasmid#44481 (Erik Jorgensen lab)
pCFJ68 (Exp5605_Punc-122::GFP::unc-54utr)	Addgene	Plasmid#19325 (Erik Jorgensen lab)
pCFJ90 (Pmyo-2::mCherry::unc-54utr)	Addgene	Plasmid#19327 (Erik Jorgensen lab)
pCFJ104 (Pmyo-3::mCherrry:::unc-54)	Addgene	Plasmid#19328 (Erik Jorgensen lab)
GFP-UtrCH (reporter for F-actin)	Addgene	Plasmid#26737 (William Bement lab)
Phic-1::mCherry	This study	pBAB# 0101
Phic-1::HIC-1::mCherry	This study	pBAB# 0102
Prab-3::HIC-1	This study	pBAB# 0103
Plet-413::HIC-1	This study	pBAB# 0104
Pmyo-3::HIC-1	This study	pBAB# 0105
Punc-17::HIC-1	This study	pBAB# 0106
Punc-25::HIC-1	This study	pBAB# 0107
Punc-17::HIC-1ΔC(4aa)	This study	pBAB# 0108
Punc-17::CWN-2::mCherry	This study	pBAB# 0109
Punc-17::NAB-1::Linker::VN173	This study	pBAB# 0110
Punc-17::HIC-1::Linker::VC155	This study	pBAB# 0111
Punc-17:: HIC-1ΔC(4aa)::Linker::VC155	This study	pBAB# 0112
Punc-17:: GFP-UtrCH	This study	pBAB# 0113
Punc-25:: GFP-UtrCH	This study	pBAB# 0114
Phic-1 ::HIC-1ΔC(4aa)::mCherry	This study	pBAB# 0115
Punc-17:: HIC-1(ΔC(4aa)+NAB-1(ABD))	This study	pBAB# 0116
Software and Algorithms
ImageJ	n/a	https://imagej.nih.gov/ij/
Prism7	Graphpad Prism	https://www.graphpad.com/
ApE	n/a	http://jorgensen.biology.utah.edu/wayned/ape/
Snapgene	Snapgene	http://www.snapgene.com/
Mind the graph	Mind the GRAPH	https://mindthegraph.com