Figure 3.

FVIII deficiency did not decrease levels of CD11b⁺cells in the spleen of Plg^−/−mice. (A) Representative HE-stained sections and immunohistochemical stains for fibrin and CD11b-expressing cells in spleens from WT, F8^−/−, Plg^−/−, and F8^−/−/Plg^−/− mice. Note the presence of fibrin deposits (black arrows) and CD11b-expressing inflammatory cells (arrowheads). Cells with megakaryocyte morphology were abundant in Plg^−/− and F8^−/−/Plg^−/− spleens (white arrows). The anti-CD11b and anti-fibrin–negative controls were spleen sections stained with an isotype antibody. Scale bar, 100 µm. The percentage of fibrin-stained tissue area (B) and percentage of CD11b-stained tissue area (C) were quantified by digital image analysis. Results are shown as individual observations and mean ± SEM and analyzed with an ANOVA with Bonferroni correction for multiple comparisons. P < .05 was considered significant; *P < .05, ***P < .001, ****P < .0001. All stained slides were scanned at 20× magnification on the Nanozoomer slide scanner (Hamamatsu Photonics K.K.), and quantified with Visiopharm Integrator System software (VIS ver. 6.7.0.2590; Visiopharm).