TABLE 2.
List of primers for quantitative PCR, semiquantitative PCR, chromatin immunoprecipitation, and RNA immunoprecipitation
| Reaction | Target | Sequencea |
|---|---|---|
| Quantitative PCR | ANRIL | F, CAACATCCACCACTGGATCTTAACA |
| R, AGCTTCGTATCCCCAATGAGATACA | ||
| E2F1 | F, ATGTTTTCCTGTGCCCTGAG | |
| R, AGATGATGGTGGTGGTGAC | ||
| SP1 | F, GCCTCCAGACCATTAAC | |
| R, GCTCCATGATCACCTGGGGCA | ||
| CTCF | F, GATCATGCACAAGCGCAC | |
| R, CCATGGTATTCCGACGTGTA | ||
| p15/CDKN2B | F, GGGAGGGTAATGAAGCTGAG | |
| R, GGCCGTAAACTTAACGACACT | ||
| p16/CDKN2A | F, GGGTCCCAGTCTGCAGTTA | |
| R, GGAGGGTCACCAAGAACCT | ||
| p21/CDKN1A | F, GCAGACCAGCATGACAGATTT | |
| R, GGATTAGGGCTTCCTCTTGGA | ||
| KLF2 | F, CTGCACATGAAACGGCACAT | |
| R, CAGTCACAGTTTGGGAGGGG | ||
| EZH2 | F, CCCTGACCTCTGTCTTACTTGTGGA | |
| R, ACGTCAGATGGTGCCAGCAATA | ||
| MYC | F, GCTGCTTAGACGCTGGATTT | |
| R, CACCGAGTCGTAGTCGAGGT | ||
| CTGF | F, CTTCTGCGATTTCGGCTCC | |
| R, TACACCGACCCACCGAAGA | ||
| VCAM1 | F, TGCCGAGCTAAATTACACATTG | |
| R, CCTTGTGGAGGGATGTACAGA | ||
| CDK6 | F, TGGAGACCTTCGAGCACC | |
| R, CACTCCAGGCTCTGGAACTT | ||
| CCND1 | F, GTTCATGGCCAGCGGGAAGAC | |
| R, CTGCCGTCCATGCGGAAGATC | ||
| 18S | F, AACCCGTTGAACCCCATT | |
| R, CCATCCAATCGGTAGTAGCG | ||
| Semiquantitative PCR | HOTAIR | F, CAGTGGGGAACTCTGACTCG |
| R, GTGCCTGGTGCTCTCTTACC | ||
| H19 | F, TGCTGCACTTTACAACCACTG | |
| R, ATGGTGTCTTTGATGTTGGGC | ||
| TUSC7 | F, GGACCTGCCCTCCATTCTAT | |
| R, GCATGGCATTTTCTCTCCTC | ||
| ANRIL | F, CAGAGCAATTCCAGTGCAAG | |
| R, GATTTGCAAAAACAGCTG | ||
| MALAT1 | F, GAATTGCGTCATTTAAAGCCTAGTT | |
| R, GTTTCATCCTACCACTCCCAATTAAT | ||
| SAF | F, CATCTCAGCCTCTTGGTGTAA | |
| R, ATGGGAGATATGGGATTGAAC | ||
| Tax | F, CCGGCGCTGCTCTCATCCCGGT | |
| R, GGCCGAACATAGTCCCCCAGAG | ||
| E2F1 | F, CACTCGGCTGACGGTGTCGTCGACC | |
| R, GGAGAGCAGGCGCAGCTGCGTAGTA | ||
| EZH2 | F, GTGGAGAGATTATTTCTCAAGATG | |
| R, CCGACATACTTCAGGGCATCAGCC | ||
| GAPDH | F, ATGGGGAAGGTGAAGGTCGGAGTC | |
| R, CCATGCCAGTGAGCTTCCCGTTC | ||
| Chromatin immunoprecipitation | ANRIL-Luc | F, GTAACCCGAATGGGGAAGCC |
| R, GCCGCTTCCTAGAAGACCAG | ||
| κB-Luc | F, TCCAGGGGAATCTCCAGG | |
| R, GTTGCTCTCCAGCGGTTCCA | ||
| CDKN1A promoter | F, CACCAGACTTCTCTGAGCCC | |
| R, AAAGGGCAACCTGATCTCCA | ||
| Myc promoter | F, GTGCATGACCGCATTTCCAA | |
| R, AATCAAAGGTGCTAGACGGG | ||
| RNA immunoprecipitation | ANRIL | F, CAACATCCACCACTGGATCTTAACA |
| R, AGCTTCGTATCCCCAATGAGATACA | ||
a
F, forward; R, reverse.