TABLE 1.
Detailed information about the prion agents used in this study
| Prion strain | Prion isolate generation | Rate of disease development |
PrPres MWa
(unglycosylated band [kDa]) |
Infectious titer (ID50/g)c |
||
|---|---|---|---|---|---|---|
| Classification | Host | Mean survival time ± the SD (attack rate [%]) |
||||
| 22L | 22L adapted to 129 Ola mice | Fast | 129 Ola mice | 128 ± 10 (100) | 21 | 1.5 × 107* |
| NTb | HuTg340 | >700 (0) | 21 | 1.5 × 107* | ||
| RML | Rocky Mountain Laboratory (RML) adapted to 129 Ola mice | Fast | 129 Ola mice | 147 ± 4 (100) | 21 | 1.6 × 107* |
| BSEm | Bovine spongiform encephalopathy (BSE) adapted to 129 Ola mice | Slow | 129 Ola mice | 142 ± 5 (100) | 19 | 1.2 × 106* |
| Fast-sCJD | Sporadic Creutzfeldt-Jakob disease type 1 from a 129MM individual adapted to 129M-HuTg340 mice | Fast | HuTg340 | 201 ± 15 (100) | 21 | 4.1 × 107† |
| NTb | 129 Ola mice | >700 (0) | 21 | 4.1 × 107† | ||
| Slow-sCJD | Sporadic Creutzfeldt-Jakob disease type 2 from a 129VV individual adapted to 129M-HuTg340 mice | Slow | HuTg340 | 507 ± 85 (100) | 21 | 1.3 × 107† |
| vCJD | Variant Creutzfeldt-Jakob disease adapted to 129M-HuTg340 mice | Slow | HuTg340 | 650 ± 60 (100) | 19 | 4.2 × 107‡ |
| C-BSE | Epidemic bovine spongiform encephalopathy (BSE) adapted to BoTg110 mice | Slow | BoTg110 | 241 ± 7 (100) | 19 | 1.9 × 107‡ |
| H-BSE | H type atypical bovine spongiform encephalopathy (BSE) adapted to BoTg110 mice | Slow | BoTg110 | 278 ± 17 (100) | 21 | NA |
a
MW, molecular weight.
b
NT, nontransmissible.
c
*, Infectious titer in Tga20 mice (transgenic mice overexpressing mouse PrP at ∼10-fold the levels expressed in wild-type mouse brain); †, infectious titer in HuTg340 mice; ‡, infectious titer in BoTg110 mice; NA, not available.