FIG 2.

Determination of chemical-mediated cytotoxicity in LLC-PK cells by MTT assay. (A to E) LLC-PK cells grown in 96-well plates were incubated with various concentrations of the indicated chemicals in triplicate for 24 h at 37°C. Afterward, the chemical-containing medium was thoroughly removed and replaced with 200 μl of MTT solution for 4 h at 37°C. Each well was incubated with 100 μl of DMSO for 10 min at room temperature. Cell viability was measured using an enzyme-linked immunosorbent assay (ELISA) reader to obtain the optical density at 570 nm.