FIG 10.

Live-cell imaging of Rhod5F rcTLPs. (A) BSC-1 cells were infected at an MOI of 10 with Rhod5F rcTLPs and imaged for 30 min. Individual particles were tracked by the signal in the 642-nm channel (left) (see Materials and Methods). Uncoating events were detected as particles with a roughly constant 642-nm signal that lose the 488-nm signal (VP7) (right). A decrease in the 561-nm intensity (VP4 Rhod5F) (right) preceded the onset of VP7 uncoating. Another example from the same experiment is shown in Fig. 9A. (B) Micrographs of all three channels of the uncoating rcTLP represented in panel A.