FIG 12.

Probability densities of 640- and 488-nm channel amplitudes from 469C and Rhod5F rcTLP binding assays. (A and B) Distribution of 640-nm amplitudes derived from particle detections as described in Materials and Methods for all 469C (A) and Rhod5F (B) binding assay samples. Blue and red arrows indicate the upper and lower limits for detection to be considered a single particle; signals weaker than those indicated by the blue arrow were taken as noise, and signals stronger than those indicated by the red arrow were considered aggregates or multiple unresolved particles. (C and D) Distribution of 488-nm amplitudes derived from particle detections for all 469C (C) and Rhod5F (D) binding assay samples (see Materials and Methods). The red arrow points to the cutoff, above which a particle was taken to be a VP7-488-coated rcTLP and below which it was considered uncoated.