FIG 5.
Us3 and UL46 synergistically promote HSV-1 reproduction during AA insufficiency. NHDFs were infected with HSV-1 Kos37 strain-derived viruses (wild type [WT], a Us3 deletion mutant [ΔUs3], a UL46 deletion mutant [ΔUL46]), or HSV-1 doubly deficient in Us3 and UL46 [ΔUs3 ΔUL46] at an MOI of 10−2. At 5.5 hpi, culture medium was replaced with AA-replete (+) or AA-free (−) RPMI 1640. At 48 hpi, cell-free lysates were prepared by freeze-thawing, and infectious virus was quantified by a plaque assay on Vero cells. (n = 4) (***, P < 0.001; ****, P < 0.0001 [as determined by 2-way analysis of variance {ANOVA} with multiple comparisons]). Error bars indicate standard errors of the means (SEM).