TABLE 1.

Primers and probes used for qPCR

Gene	Primer or probea	Sequence (5′–3′)b	Concentration (nM)	Reference
JEV	PF	GGTGTAAGGACTAGAGGTTAGAGG	400	74
	PR	ATTCCCAGGTGTCAATATGCTGTT	400
	Probe	FAM-CCCGTGGAAACAACATCATGCGGC-TAMRA	100
18S	PF	CGCCGCTAGAGGTGAAATTC	400	75
	PR	GGCAAATGCTTTCGCTCTG	400
	Probe	FAM-TGGACCGGCGCAAGACGGA-TAMRA	100
pIL-6	PF	CTGGCAGAAAACAACCTGAACC	400	This study
	PR	TGATTCTCATCAAGCAGGTCTCC	400
	Probe	FAM-TGGCAGAAAAAGACGGATGC-TAMRA	100
pIL-8	PF	CCGTGTCAACATGACTTCCAA	500	76
	PR	GCCTCACAGAGAGCTGCAGAA	500
	Probe	FAM-TTCTTCGCCCTCAGTGTGAA-TAMRA	125
IFN-λ 3	PF	GCCAAAGATGCCTTAGAAGAG	500	77
	PR	CAGAACCTTCAGCGTCAGG	500
	Probe	FAM-CGCGATCGCAAGTGCCGCTCCCGCCTCTGATCGCG-TAMRA	125
SOCS1	PF	TTCTTCGCCCTCAGTGTGAA	500	78
	PR	GGCCTGGAAGTGCACGC	500
	Probe	FAM-TTCGGGCCCCACAAGCATCC-BHQ1	125
CCL2	PF	CCATCAGCTCCCACACCGAA	500	This study
	PR	AAGGACCTGGGTGCAGAAGG	500
	Probe	FAM-TGCAGCCCTCCTGTGCCTGCTGC-TAMRA	125
CCL5	PF	TCCATGGCAGCAGTCGTCTT	500	This study
	PR	CAGGCTCAAGGCTTCCTCCA	500
	Probe	FAM-ACCGCCAGGTGTGTGCCAACCCAGA-TAMRA	125
CXCL10	PF	TTGAAATGATTCCTGCAAGTCAA	500	79
	PR	GACATCTTTTCTCCCCATTCTTTT	500
	Probe	FAM-CTTGCCCACATGTTGAGATCATTGCCAC-TAMRA	125

^aPF, forward primer; PR, reverse primer.

^bFAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.