FIG 2.

Antibody response in goats to PPRV vaccines. Goats were vaccinated with N75 or S96, and serum samples were taken at 0, 7, 14, 21, and 28 dpv. Sera taken at all time points were assayed for antibodies to the PPRV N protein (a) and the PPRV H protein (b) using available cELISA kits. Sera taken at 14, 21, and 28 dpv were additionally assayed for neutralization of PPRV; neutralizing titers were determined against N75 vaccine virus (c) and S96 vaccine virus (d). (e) The differences in titers (titer against N75 virus – titer against S96 virus) for each individual serum was calculated, and these differences are plotted. Note that the manufacturer’s recommended calculation for the N protein cELISA kit (in panel a) gives values that decrease as the amount of anti-N antibody increases (percentage of negative control), whereas that for the H protein cELISA kit (in panel b) gives values that increase as the amount of competing antibody increases (percent inhibition of binding of MAb). The data are presented as box-and-whisker plots, in which the bars span the minimum and maximum values, and the box shows the range from the first to the third quartile. The central horizontal line in each box shows the median value. The numbers of samples assayed at each time point shown were as follows: 25/19/25/19/24 (a), 15/10/15/10/14 (b), and 15/15/15 (c to e).