Figure 6.

MSP-1^1282–1301 analogue-HLA-DRβ1∗ alleles and red blood cell-binding patterns. A. The relative values for an analyzed peptide binding to a given HLA-allele were determined. Biotinylated peptides used for binding assays were HA: HA306-318; and GFK(A)₇. Values shown in bold correspond to residues providing efficient binding to a given allele. Values higher than 50% are considered significant and are shaded. B. On the center pannel, specific binding activity of ¹²⁵I-all-L 1585 (MSP-1^1282–1301) peptide and its D-partial substituted analogs to Red Blood Cells (RBCs) in concentrations ranging from 0 to 200 nM; and MSP-1^1282–1301 (represented by solid bars). Native peptide RBC-binding inhibition by D-partial substituted analogs is shown by gray bands on the right-hand side; ND is used for non-determined and 1592 control* is used for the 1592 (MSP-1^1422–1441) non-binding peptide.