Skip to main content
. 2011 Jan 18;16(1):738–761. doi: 10.3390/molecules16010738

Figure 2.

Figure 2

(a) Enzymatic activity. sPLA2-Nar showed a significant decrease compared to native sPLA2 and was similar to sPLA2 treated with 4-bromophenacyl bromide (sPLA2-p-BPB) (n = 12, *P < 0.05). (b) Measurement of creatine kinase (CK) activity from samples of animals treated with saline (negative control), native sPLA2 (50 μL, 50 μg), or (sPLA2-Nar; 50 μL, 50 μg). The last bar represents the effect of sPLA2 treated with p-BPB (50 μL, 50 μg). Results of creatine kinase levels were expressed as units of enzymatic activity per liter (U/L) of blood. Each column represents six animals, and error bars indicate the S.E.M. *P < 0.05 compared with sPLA2 native. (c) Neurotoxic effect of sPLA2 and sPLA2-Nar on the chick biventer cervicis preparation. Results were expressed as a percent modification of twitch tension, and each point represents the mean ± S.E.M. of five preparations. * P < 0.05. (d) The washed platelets assay was performed with venous blood collected from healthy volunteers. The blood was centrifuged in 3.8% trisodium-citrate for 10 minutes at 200 g, and the residue was centrifuged again for 20 minutes at 1,500 g. Aggregations were performed using native sPLA2 and sPLA2-Nar.