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. 2010 Oct 18;15(10):7197–7207. doi: 10.3390/molecules15107197

Figure 4.

Figure 4

The inhibitory effect of siRNA2 on SARS-CoV M gene expression. (a) RT-PCR analysis on the dose effect of si-M2 on M mRNA expression. About 1 μg of pCMV-Myc-M was co-transfected with increased doses of si-M2. The reaction products were subjected to RT-PCR analysis using SARS-M and GAPDH primers. (b) Quantitation of the RT-PCR results in (a). (c) si-M2 specifically inhibited pEGFP-M fusion gene but not pEGFP gene expression. About 1 μg of either pEGFP-M or pEGFP was co-transfected with increased doses of si-M2. (d) si-M2 but not si-IL-17RE effectively inhibited pEGFP-M gene expression. About 1 μg of pEGFP-M plasmid was co-transfected with increased doses of either si-M2 or si-IL17RE. (e) Flow cytometric analysis on the intensity of pEGFP-M gene expression in (d).