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Application of ImageJ toolbox to measure osteoclast activity from TRAP stained sections. Rat vertebral sample was used to perform TRAP enzyme histochemistry. Osteoclasts are identified as multi-nucleated TRAP positive cells near the bone surface. The scale was set up before proceeding with measurements. The length of ruffled borders (arrows) govern the osteoclast activity. The length of ruffled border was measured using free-hand line tool after the scale was set.
