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. 2017 Aug 21;38(11):1979–1992. doi: 10.1177/0271678X17726062

Figure 5.

Figure 5.

Neuronal death and activation of microglia and astrocytes after CA/CPR. (a–d) Representative photomicrographs of immunohistochemistry for NeuN, MAP2, Iba-1, and GFAP in the hippocampal CA1 region of the sham group and the experimental groups at seventh day after ROSC. All images were captured at × 400 magnification. Scale bar indicates 100 μm, n = 5 for sham group, and n = 20 for experimental groups. (e–h) Semi-quantitative results of NeuN, MAP2, Iba-1, and GFAP, respectively. NeuN staining cells are reported as positive number, while MAP2, Iba-1, and GFAP staining as the relative intensity of staining (percent) of the area. Data are presented as mean ± SD. Sham: sham group; CA: cardiac arrest group; HAase: hyaluronidase group; HC: hydrocortisone group; CPR: cardiopulmonary resuscitation; NeuN: neuronal nuclei; MAP2: microtubule-associated protein 2; Iba-1: ionized calcium-binding adapter molecule 1; GFAP: glial fibrillaryacidic protein; ROSC: return of spontaneous circulation; SD: standard deviation. *p < 0.05 vs. sham group. #p < 0.05 vs. CA group. + p < 0.05 vs. HC group.