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. 2018 Nov 21;5:322. doi: 10.3389/fmed.2018.00322

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Copyright © 2018 Bucur and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Imaging regular/expanded kidney tissue sections with inverted fluorescence microscope. (A) The configuration of the objective and the regular kidney specimen on a glass slide with a water-immersion objective (optional, air objective or oil objective can also be used for imaging) for pre-expansion imaging. (B) Photo of the typical setup on an inverted microscope, as depicted in (A). (C) The configuration of the objective and the expanded sample on an inverted microscope with a water-immersion objective (optional, air objective or oil objective can also be used for imaging) for post-expansion imaging. (D) Photo of the typical setup on an inverted microscope, as depicted in (C).