Table 1.
Intra-assay and inter-assay accuracy and precision tests for AS and DHA in human plasma.
| Theo. Conc . | Found (n = 6) | Inaccuracy (%) | %CV | |||||
|---|---|---|---|---|---|---|---|---|
| AS | DHA | AS | DHA | AS | DHA | AS | DHA | |
| Intra-assay | ||||||||
| 1. LLOQ | 3.20 | 5.33 | 3.02 | 5.53 | -5.73 | 3.66 | 17.6 | 7.15 |
| 2. 3 x LLOQ | 10.0 | 16.0 | 10.7 | 16.3 | 7.12 | 1.71 | 2.76 | 5.29 |
| 3. Medium | 500 | 800 | 533 | 796 | 6.62 | -0.55 | 4.00 | 5.28 |
| 4. 0.8 x ULOQ | 2,500 | 4,000 | 2,481 | 3,810 | -0.77 | -4.74 | 1.68 | 3.11 |
| 5. ULOQ | 3,000 | 5,000 | 2,782 | 4,637 | -7.28 | -7.27 | 3.78 | 4.10 |
| Inter-assay | ||||||||
| 1. LLOQ | 3.20 | 5.33 | 3.28 | 5.65 | 2.36 | 5.93 | 5.44 | 10.2 |
| 2. 3 x LLOQ | 10.0 | 16.0 | 9.93 | 16.7 | -0.69 | 4.25 | 8.88 | 5.19 |
| 3. Medium | 500 | 800 | 496 | 785 | -0.76 | -1.82 | 7.98 | 5.87 |
| 4. 0.8 x ULOQ | 2,500 | 4,000 | 2,445 | 3,872 | -2.21 | -3.20 | 5.48 | 4.89 |
| 5. ULOQ | 3,000 | 5,000 | 2,912 | 4,966 | -2.93 | -0.68 | 5.03 | 5.24 |
Values are average of six replicates for intra-assay test and triplicates per day for six different days of inter-assay test.