Figure 4.

miR-339-5p arrests cell cycle of lung cancer cells following IR exposure. (A) A549 and H460 cells were non-transfected (control) or transfected with NC or miR-339-5p mimics. Twenty-four hours later, cells were exposed by 12 Gy of IR or untreated for another 24 h. Cell cycle was analyzed by flow cytometry. MiR-339-5p caused a G0/G1 arrest. (B) Quantification for cell cycle attribution. The percentage of cell population at G0/G1, S, and G2/M phases are represented as mean ±SD (* P<0.05). (C) After transfection, the H460 cells were plated into 6-well plates. With/without radiation (12 Gy), cells clones were stained by crystal violet and were calculated 10 days later.