Figure 6.

miR-339-5p sensitizes lung cancer cells to IR by targeting PRL-1. (A) The expression levels of PRL-1, Bax, and Bcl-2 under 0Gy/8Gy were detected by Western blotting in H460 cells after indicated transfections. β-actin was used as a loading control. (B) Quantification of Western blotting results as in (A). (C) Cell apoptosis was detected by annexin V/PI staining and flow cytometry in indicated cells. (D) Mean apoptosis rate assessed by flow cytometry in response to IR exposure as in (C). Data are expressed as the mean ±SD (* P<0.05).