Figure 5.

Analysis of the carbohydrate specificity of tepary bean hemagglutinin by lectin affinity chromatography. A glycopeptide mixture purified from thermolysin-treated thyroglobulin was applied to a column with a resin containing immobilized PAA. The glycopeptides were eluted in three fractions. Fraction 1 contains the unbound glycopeptides. Fractions 2 and 3 are the fractions that were retarded by the lectin column and that therefore have affinity for the lectin. Carbohydrate composition of these fractions is presented in Table 8. The proposed structure of the oligosaccharide with the highest affinity with the tepary bean lectin is presented in Figure 6.