Table 4.
Results of the sequence obtained by LC/MS/MS on tryptic peptides of proteins excised from 2D-PAGE separation of the tepary bean subunits. The calculated masses were obtained from a theoretical tryptic digest of the sequence of the lectin precursor from Phaseolus acutifolius reported in the NCBI protein sequence data base (Accession No gi|1086123).
| Calculated Mass | Observed Mass | Start residue | End residue. | Sequence |
|---|---|---|---|---|
| 1095.6156 | 1095.6118 | 161 | 170 | HIGIDVNSIK |
| 1317.7776 | 1317.7792 | 197 | 208 | LLVASLVYPSQK |
| 1324.6995 | 1324.7072 | 209 | 220 | TSFIVSDTVDLK |
| 1635.8125 | 1635.7968 | 140 | 153 | AHTVAVEFDTLYNR |
| 1800.9086 | 1800.8922 | 56 | 72 | LTNLNDNGEPTLSSLGR |
| 2219.2183 | 2219.2234 | 102 | 124 | VPNNAGPADGLAFALVPVGSKPK |