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. 2018 Nov 13;476(12):2442–2453. doi: 10.1097/CORR.0000000000000548

Fig. 3.

Fig. 3

IL1β protein secretion depends on the NLRP3 inflammasome and adherent PAMPs. Wild-type and NLRP3-/- macrophages were treated with a vehicle control (blue triangles), endotoxin-free titanium particles (green squares), or titanium particles with adherent bacterial debris (red circles) for 8 hours. IL1β was measured in supernatant using ELISA. All values are shown as percent of maximal IL1β secretion in that experiment, which are the wild-type macrophages stimulated by titanium particles with adherent bacterial debris. N = 3 independent experiments were performed. Each experiment included triplicate cell culture wells per group, each assayed in triplicate. Error bars represent SD. Statistical significance was determined by one-sided analysis of variance. ***p < 0.001. Ti = titanium; NLRP3 = Nod-like-receptor-protein-3.