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Flow chart illustrating progression of features evolving within 4 × 6 TM cation channels, including the three primary voltage-gated channels contained in single cell choanoflagellates (Nav2, Cav1, and Cav3) (pink color, 3 genes) and their diversification and specialization within protostome invertebrates (green color, 6 genes) and vertebrates (purple color, 21 genes).
