Figure 3.

KDM4B inhibition significantly reduces the Aa-LPS-induced immune response in primary macrophages. Male and female murine bone marrow derived macrophages were pre-treated for 1h with the selective KDM4B inhibitor, ML324, a family-wide KDM4 inhibitor, JIB-04, and a KDM1A inhibitor, GSK-LSD1 or DMSO vehicle. Following drug treatment, cells were challenged with Aa-LPS for 8 and 24h, where gene expression and supernatant protein concentration were measured via rt-qPCR relative to GAPDH and ELISA relative to a standard curve. Data was normalized as a percentage of the maximal response (red) in each group for display. Refer to figure S1 for raw data and statistical significance. n = 4 per experiment, data is representative of 3 experiments.