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. 2018 Nov 23;10:6195–6204. doi: 10.2147/CMAR.S184180

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© 2018 Zhou et al. This work is published and licensed by Dove Medical Press Limited

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Glucose deprivation enhances the autophagy in HeLa cell induced by S1.

Notes: HeLa cells were treated with 10 µM S1, EBSS, and 10 µM S1 and EBSS for 8 hours. (A) The expression of LC3-II, P62, Beclin 1, and Atg12–5 was determined by Western blot. (B) Quantification of P62 and LC3-II, Beclin 1, and Atg12–5 levels. Data are presented as mean ± SD (n=3). *P<0.05 vs control group. ^#P<0.01 vs control group. (C) The expression of LC3 was detected by confocal microscopy (bar, 10 µm). (D) Lysosome was observed with LysoTracker staining by confocal microscopy (bar, 10 µm).

Abbreviation: EBSS, Earle’s balanced salt solution.