Skip to main content
. 2018 Nov 23;10:6195–6204. doi: 10.2147/CMAR.S184180

Figure 6.

Figure 6

Autophagy inhibitor CQ increases S1-induced apoptosis in HeLa cell.

Notes: The 50 µM CQ (an inhibitor of autophagy) was used to pretreat the HeLa cell for 30 minutes, and then, HeLa cells were treated with 10 µM S1, EBSS, and 10 µM S1and EBSS for 8 hours. (A) Cell viability was determined by MTT assay in each group. Data are presented as mean ± SD (n=3). *P<0.05 vs control group. #P<0.01 vs control group. (B) The expression of LC3-II and P62 was determined by Western blot. (C) The expression of Cyto C, cleaved caspase-3, and cleaved PARP-1 was determined by Western blot. (D) Quantification of P62 and LC3-II levels. (E) Quantification of Cyto C, cleaved caspase-3, and cleaved PARP-1 levels. (F) Lysosome was observed with LysoTracker staining by confocal microscopy (bar, 10 µm). Data are presented as mean ± SD (n=3).

Abbreviations: CQ, chloroquine; EBSS, Earle’s balanced salt solution.