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. 2018 Sep 13;115(8):1457–1469. doi: 10.1016/j.bpj.2018.09.003

Figure 1.

Figure 1

Imaging individual collagen proteins. (A) A schematic of triple-helical collagen. (B–E) Representative AFM images of each of the four collagen samples investigated in this work. For each sample, collagen was deposited from a solution of 100 mM KCl + 1 mM HCl. Scale bars, 250 nm. (B) Rat tail-derived type I collagen. (C) Recombinant human type I collagen. (D) Cartilage-derived human type II collagen. (E) Recombinant human type III collagen. (F) SmarTrace starts with user input points along a collagen chain (left image). Initial splines connecting the three input points (dashed lines) do not follow the chain. The SmarTrace algorithm identifies both the center line of the chain (red) and its width (blue—used for chain tracing but no further analysis), as shown in the right image. To view this figure in color, go online.