Figure 3.

Overexpression of miR-27a attenuated LPS-induced inflammatory response. Groups of mice were given agomir-27a or agomir NC (2 mg/kg) by tail intravenous injection 24 h prior to 1 mg/kg LPS treatment. The mice were sacrificed after LPS administration for 24 h, and then the BALF were collected for analysis. (a,b) The total counts of cells and neutrophils from the BALF were counted using a hemocytometer (n = 3/group). (c) MPO activity in lung tissues was measured at 24 h after LPS challenge (n = 3/group). (d-f) IL-6, IL-1β and TNF-α levels in BALF were measured at 24 h after LPS challenge (n = 3/group). Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 vs. control group. ##p < 0.01 vs. LPS alone group.