Figure 4.

Overexpression of miR-27a inhibited LPS-induced apoptosis. Groups of mice were given agomir-27a or agomir NC (2 mg/kg) by tail intravenous injection 24 h prior to 1 mg/kg LPS treatment. The mice were sacrificed after LPS administration for 24 h, and then the BALF were collected for analysis. (a) Apoptotic cells in the tissues were observed and the number of TUNEL positive cells was counted in 5 to 10 fields for each slide (n = 3/group). (b) The protein expression levels of Bcl-2, Bax and cleaved-caspase 9 were detected by Western Blot (n = 3/group). Data represent the mean ± SD of three independent experiments. *p <  0.05, **p < 0.01 vs. control group. ##p < 0.01 vs. LPS alone group. (C) The bands were semi-quantitatively analyzed by using Image J software, normalized to β-actin density. Data represent the mean ± SD of three independent experiments. *p  <  0.05, **p < 0.01 vs. control group. ##p < 0.01 vs. LPS + agomir-NC group.