Table 2.

The Melting Temperature T_m Values for 20 dsRNAs in 1 M [Na⁺] Solution

	Sequencea	Length (bp)	Expt. (°C)d	Pred. (°C)	Deviation (°C)
1	CGCG	4	19.9	19.5	−0.4
2	GGCC	4	34.3	35.1	+0.8
3	GCGC	4	26.6	28.1	+1.5
4	CCGG	4	27.2	26.6	−0.6
5	CGCGCG	6	57.8	58.5	+0.7
6	CCGCGG	6	59.8	61.2	+1.4
7	CCAUGG	6	46.4	45.3	−1.1
8	GUCGAC	6	45.3	46.8	+1.5
9	AGCGCU	6	50.2	51.1	+0.9
10	UCAUGA	6	27.2	25.7	−1.5
11	GAGGAG	6	50.9	52.0	+1.1
12	AACUAGUU	8	45.7	45.5	−0.2
13	ACUUAAGU	8	40.3	41.3	+1.0
14	ACCUUUGG	8	56.3	54.8	−1.5
15	GCCAUGGC	8	71.4	69.5	−1.9
16	GCUGCGAC	8	67.9	65.8	−2.1
17	CCAUAUGG	8	57.0	59.1	+2.1
18	GAACGUUC	8	52.3	53.6	+1.3
19	AAGGUUGGAA	10	66.5	64.1	−2.4
20	AUUGGAUACAAAb	12	55.4	58.1	+2.7
21	AAAAAAAUUUUUUUc	14	33.2	35.0	+1.8
22	CCUUGAUAUCAAGG	14	76.7	74.3	−2.4

^aThere is only one sequence for dsRNA, and the other is complementary to the shown one. The strand concentrations are 0.1 mM.

^bThe strand concentration of AUUGGAUACAAA is 8 μM.

^cThe strand concentration of AAAAAAAUUUUUUU is 3.9 μM.

^dThe experimental data are from (66, 70, 71, 72).