Fig. 3.

(+)-Terrein suppresses IL-6/sIL-6R-induced phosphorylation of JAK1, Akt, and SHP-2 in HGFs. HGFs (5.0 × 10⁴ cells/cm²) were cultured until they reached subconfluence. Next, the cells were pretreated with (+)-terrein (10 μM) for 30 min, followed by stimulation with IL-6/sIL-6R (50 ng/mL each) for 5 min. After stimulation, total cell lysates were collected. Phosphorylation of JAK1 (A), Akt (B), and SHP-2 (C) were determined by Western blotting using phospho-specific antibodies, respectively. Relative band density of each phosphorylation level is compared with β-actin using Image J software. β-Actin was detected by reprobing the blots after detecting SHP-2. Data are expressed as mean ± SD and are representative of three independent experiments; *p < 0.05 (ANOVA/Scheffe's test). Representative full, non-adjusted blot images are shown as supplementary figure (file name: 20181115supplementaryfigure_Heliyon_YamamotoS.pdf).