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. 2018 Oct 1;159(11):3860–3873. doi: 10.1210/en.2018-00608

Table 1.

Primers Used in qRT-PCR Studies

Symbol Reference mRNA Forward Primer Reverse Primer Amplicon
Kiss1 NM_181692.1 26F-TGCTGCTTCTCCTCTGTGTG 174R-AGGCTTGCTCTCTGCATACC 149 bp
Kiss1r NM_001301151 502F-CTGGGAGACTTCATGTGCAA 614R-GGGAACACAGTCACGTACCA 113 bp
Esr1 NM_012689.1 44F-TTCGAGCACATTCCTTCCTT 235R-GCTTTGGTGTGAAGGGTCAT 191 bp
Esr2 NM_012754 1101F-GAAGCTGAACCACCCAATGT 1310R-CAGTCCCACCATTAGCACCT 209 bp
Erk2 NM_053842 274F-GCCGCGCTACACTAATCTCT 464R-TGCCGATGATGTTCTCATGT 190 bp
Fosl2 NM_001013146 107F-CTCCCGAAGAGGAGGAGAAG 332R-GGACTGATTTTGCACACAGG 226 bp
JunB NM_021836 1148F-TGGAGGACAAGGTGAAGACA 1436R-GCCAGAGTCCAGTGTGTGAG 289 bp
Rn18s NR_046237.1 1622F-GCAATTATTCCCCATGAACG 1744R-GGCCTCACTAAACCATCCAA 123 bp