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Primers for Conventional RT-PCR to Detect Kiss1 Transcript Variants
| Exon | Chromosome Location | Forward Primer | Reverse Primer | Amplicon |
|---|---|---|---|---|
| Exon 2-3 | Ch13: 50529847-50532103 | 2F-ATGATCTCGCTGGCTTCTTG | 3R-CTTTTGCCAGGCATTAACGA | 147 bp |
| Exon 1-3 | Ch13: 50529847-50535078 | 1F-CTCAGTGTGCTCCAACTACC | 3R-CTTTTGCCAGGCATTAACGA | 322 bp |
| 247 bp |
Abbreviation: Ch, chromosome.
