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. 2018 Sep 14;7(1):73–91. doi: 10.1016/j.jcmgh.2018.09.003

Figure 4.

Figure 4

Morphological characteristics and functional analysis of cell line–derived ESCC 3D organoids. ESCC 3D organoids were grown with TE11 cells in indicated cell culture media to acquire (A) phase contrast images and evaluate (B) organoid growth (size) at indicated time points. (C) Organoid formation rate was determined at day 11. RPMI1640 and DMEM are supplemented with 10% FBS. aDMEM/F12+ is fully supplemented as used to grow patient-derived 3D organoids. Scale bar =100 μm. (B) Data are presented as mean ± SD of at least 7 organoids. Data represent 3 independent experiments with similar results and 1-way analysis of variance with multiple comparisons (Tukey) was performed for each (B) time point in and (C) condition. *P < .05 vs RPMI1640, n = 3. (D) 3D organoids were grown with indicated ESCC cell lines in RPMI-10% FBS for morphological analyses by H&E staining. All tested cell lines give rise to solid 3D organoids comprising atypical cells with high nuclear/cytoplasmic ratio. Organoids from TE4, TE9 and TE10 show more basophilic cells with moderate to severe atypia. TE11 organoids show more eosinophilic cytoplasm and little nuclear atypia. TE8 and TE5 organoids represent intermediate variants with moderate atypia and eosinophilic cytoplasm. Scale bar = 100 μm.