Table 2.
3D Organoids Formation Frequency per Organoid Size From ESCC Patients
| Patient | Normal Mucosa-Derived 3D Organoids |
Tumor-Derived 3D Organoids |
||||
|---|---|---|---|---|---|---|
| 50–100 μm | 100–200 μm | ≥200 μm | 50–100 μm | 100–200 μm | ≥200 μm | |
| 1 | 0 | 0 | 0 | 22 ± 11 | 4 ± 1.3 | 0 |
| 4 | 43 ± 6.7 | 25 ± 5.0 | 8 ± 2.6 | 70 ± 12 | 26 ± 6.5 | 7 ± 3.2 |
| 5 | 9 ± 1.3 | 0.8 ± 0.4 | 0 | 78 ± 12 | 39 ± 7.5 | 0 |
| 8 | 56 ± 12 | 15 ± 4.0 | 0 | 22 ± 3.2 | 9 ± 2.4 | 0 |
| 9 | 52 ± 1.5 | 28 ± 1.7 | 0 | 0 | 0 | 0 |
| 10 | 27a | 11a | 0 | 31 ± 3.2 | 33 ± 5.4 | 2.1 ± 0.7 |
| 12 | 25 ± 6.1 | 13 ± 4.0 | 0 | 105 ± 7.8 | 58 ± 3.3 | 0.3 ± 0.2 |
| 15 | n.d. | n.d. | n.d. | 93 ± 20 | 4 ± 1.8 | 0 |
| 16 | n.d. | n.d. | n.d. | 113 ± 8.7 | 16 ± 2.8 | 1.1 ± 0.4 |
| 17 | >200 | 237 ± 56 | 4 ± 0.3 | 0 | 0 | 0 |
| 18 | 120 ± 5.3 | 74 ± 3.8 | 3 ± 0.7 | 83 ± 6.4 | 56 ± 4.5 | 21 ± 1.9 |
| 19 | >200 | 92 ± 4.3 | 8 ± 1.2 | 84 ± 17 | 55 ± 9.2 | 22 ± 4.5 |
| 20 | >200 | 107 ± 5.4 | 4 ± 0.8 | >200 | 27 ± 4.2 | 0.2 ± 0.1 |
NOTE. Values are mean ± SD. The 3D organoid formation rate from ESCC patients was determined at day 14 as the average number of organoids formed from 2 × 104 cells seeded per well. 3–10 independent wells were used except patient 10.
n.d., not determined (due to a small number of viable cells isolated from a biopsy).
a
Single well only for 3D organoids from normal mucosa.