Table 3.

Frequency of Organoid Types Within Patient Tumor-Derived 3D Organoids

Patient	Tumor-Derived 3D Organoids Content
	Non-Neoplastic (%)	Neoplastic (SCC-Compatible) (%)
1	n.d.	n.d.
4	28	72
5	100	0
8	95	5
10	86	14
12	100	0
15	n.d.	n.d.
16	67	33
18	100	0
19	92	8
20	87	13
23	25	75
24	67	33
26	11	89

NOTE. The primary 3D organoids (P0) generated directly from ESCC and OPSCC patient biopsies were evaluated by H&E staining for the frequency of 3D structures with non-neoplastic and neoplastic characteristics. Each organoid type was determined by evaluating 15–119 spherical structures (>50 μm) for each patient except patient 16 where only 3 3D organoid structures were detected throughout all H&E slides examined. None of the 3D organoids from normal mucosa contained neoplastic organoids.

n.d., not determined.