Table 1.
Plasmids and strains used in the study.
| Plasmids | Characteristics | Source |
|---|---|---|
| pBBR1MCS-2 | Plasmid vector; kanr | Addgene |
| pGEM-3Zf(+) | Plasmid vector; ampr | Promega |
| pBR322 | Plasmid vector; tetr | Promega |
| pGEMldh | pGEM-3Zf(+) derivative, carrying a 1.32 kb fragment of PCR-amplified genomic DNA, representing the major part of the ORF of respiratory D-lactate dehydrogenase (ldh; ZZ6 RS04930), cloned between BamHI and HindIII sites of the MCS | Present work |
| pGEMldh::tetr | pGEMldh derivative, carrying a 1.49 kb fragment of PCR-amplified DNA, corresponding to the tetracycline resistance determinant of pBR322, inserted in the AgeI site of ldh | Present work |
| pBBRgap | pBBR1MCS-2, containing an 0.2 kb fragment of the chromosomal DNA, corresponding to the promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (ZZ6 RS05365), cloned between the SacI and BamHI sites of the MCS | Present work |
| pBBRgapldh | pBBRgap derivative, carrying a 1.73 kb fragment of PCR-amplified genomic DNA with the ORF of ldh, cloned between BamHI and HindIII sites of the MCS, next to the gap promoter | Present work |
| Strains | ||
| ndh- | Zm6 strain with a cmr insert in the ORF of respiratory type II NADH dehydrogenase gene (ndh) | Kalnenieks et al. (2008) |
| ndh-ldh- | ndh- strain with a tetr insert in the ORF of respiratory D-lactate dehydrogenase gene (ldh) | Present work |
| ndh-ldh+ | ndh- strain, transformed with pBBRgapldh | Present work |
| ndh-ldh-ldh+ | ndh-ldh- strain, transformed with pBBRgapldh | Present work |