Table 3.
Lactate:CoQ1 oxidoreductase and lactate oxidase activity, U (mg prot)−1, and relative transcript levels of ldh in the mutant strains.
| Strain: | ndh- | ndh-ldh+ | ndh-ldh- | ndh-ldh-ldh+ |
|---|---|---|---|---|
| Lactate: CoQ1 oxidoreductase activity (in membranes) | 0.039 ± 0.003 | 0.099 ± 0.006 | 0.000 ± 0.001 | 0.028 ± 0.002 |
| Lactate oxidase activity (in cell-free extracts) | 0.082 ± 0.020 | 0.112 ± 0.042 | 0.000 ± 0.004 | 0.068 ± 0.002 |
| RT-qPCR log2<mutant/ndh-> | – | 3.3 | − 8.1 | − 0.4 |