Table 2.
Central composite design for the decoction: temperature and time, with observed responses (total phenolic content, DPPH scavenging activity, and crude Pelargonium graveolens polysaccharide yield).
TEST | Factors | Responses | |||||
---|---|---|---|---|---|---|---|
X1 | X2 | Y1 | Y2 | Y3 | |||
| |||||||
Temperature (°C) | Coded level | Time (min) | Coded level | Total phenolic content (mg GAE/gDM) | DPPH scavenging activity (%) | CPGP yield (%) | |
| |||||||
1 | 81 | -1 | 10 | -1 | 28.24 | 72.9 | 3.48 |
| |||||||
2 | 95 | 1 | 10 | -1 | 36.04 | 81.93 | 8.97 |
| |||||||
3 | 81 | -1 | 18 | 1 | 30.37 | 65.13 | 4.11 |
| |||||||
4 | 95 | 1 | 18 | 1 | 36.36 | 85.92 | 7.78 |
| |||||||
5 | 88 | 0 | 14 | 0 | 30.79 | 68.7 | 7.25 |
| |||||||
6 | 88 | 0 | 14 | 0 | 33.8 | 77.2 | 4.99 |
| |||||||
7 | 78 | -1.41 | 14 | 0 | 27.05 | 60.5 | 4.06 |
| |||||||
8 | 98 | 1.41 | 14 | 0 | 36.54 | 88.44 | 8.21 |
| |||||||
9 | 88 | 0 | 8 | -1.41 | 32.27 | 79.41 | 5.64 |
| |||||||
10 | 88 | 0 | 20 | 1.41 | 32.4 | 76.47 | 7.33 |
| |||||||
11 | 88 | 0 | 12 | -0.5 | 31.64 | 73.11 | 4.68 |
| |||||||
12 | 88 | 0 | 16 | 0.5 | 31.63 | 83.19 | 5.48 |
| |||||||
13 | 84 | -0.5 | 14 | 0 | 26.86 | 67.65 | 3.69 |
| |||||||
14 | 92 | 0.5 | 14 | 0 | 34.42 | 81.3 | 5.95 |
| |||||||
15 | 79 | -1.3 | 12 | -0.5 | 28.47 | 65.69 | 3.43 |
| |||||||
16 | 97 | 1.3 | 16 | 0.5 | 35.54 | 83.82 | 6.93 |