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. 2018 Nov 26;4(11):e00971. doi: 10.1016/j.heliyon.2018.e00971

Fig. 9.

Fig. 9

PI3K is implicated in IL-33-induced Akt activation and GSK-3β inactivation. PC-12 cells, transfected with the NC siRNA or the PI3K siRNA, were treated with IL-33 (1 ng/mL) for 30 min, followed by Western blotting using antibodies against Akt, pT308-Akt, pS473-Akt, GSK-3β, pS9-GSK-3β, and pY216-GSK-3β. (A) (D) Western blot images. The arrow shown in (D) indicates the signal band at 46 kDa for pY216-GSK-3β. (B) (C) In the graphs, each column represents the mean (±SEM) signal intensity for pT308-Akt and pS473-Akt relative to the signal intensity for Akt (n = 8 Western blot results from independent experiments). (E) (F) In the graphs, each column represents the mean (±SEM) signal intensity for pS9-GSK-3β and pY216-GSK-3β relative to the signal intensity for GSK-3β (n = 8 Western blot results from independent experiments). P values shown in the graphs, ANOVA followed by a Bonferroni correction.