Figure 4.

Drug responses of liposarcoma PDCs.

(A) Each response hit is represented as a box graph. Dose-response analyses were carried out using GraphPad Prism 6 software program (detailed in Materials and Methods). Each column represents drugs, which are clustered by color according to targeting pathway or molecules. Bar graphs represent the hits for the AUC (y-axis). (B). Comparison of the IC50 of drugs screened in PDCs. On each graph, the y-axis represents the cell survival (%) of the indicated drugs. (C) Proteasome inhibitor exhibited cytotoxicity in PDCs. A liposarcoma cell line and PDCs were treated with vehicle or bortezomib (0-80 nM) for 48 hours. The effects of these drugs on proliferation were assayed by a CCK8 experiment. The IC50 values of both drugs were calculated. The y-axis represents the percentage of cells under each condition. Each point was analyzed in triplicate. (D) Western blot analysis of GS11-079 PDC and LPS246 cell line. Cells were treated with bortezomib (0-10 μM) for 48 hours. Lysates were subjected to an analysis of the efficacy of bortezomib in inhibiting the proteasomal degradation pathway.