Table 1.
Use of lambda-display libraries for antigen discovery and diagnostic applications.
| Organism | Applications | Libraries | Results | References |
|---|---|---|---|---|
| Toxoplasma gondii | Antigen discovery | Whole parasite cDNA and stage-specific gene collections | Identification of a large panel of antigens containing B- and T-cell epitopes | [38,46,47] |
| Toxoplasma gondii | Development of diagnostic immunoassays | Whole parasite cDNA and stage-specific gene collections | Assay prototypes for diagnosis of congenital toxoplasmosis in pregnant women and infants | [63,64,65] |
| Toxoplasma gondii | Development of DNA-based vaccines | Stage-specific gene collections | DNA vaccines conferring protective immunity against chronic toxoplasmosis | [48,66,68] |
| Streptococcus pneumoniae | Antigen discovery for vaccine development | Genomic DNA | Identification of a large panel of immunodominant antigens | [52] |
| Streptococcus pneumoniae | Epitope mapping | Genomic DNA | Isolation and characterization of streptococci conserved epitopes | [53] |
| Mycoplasma pneumoniae | Antigen discovery for diagnostic applications | Genomic DNA | Isolation and characterization of B-cell regions for diagnostic immunoassays | [56,69] |
| Human cytomegalovirus (HCMV) | Antigen discovery | Genomic DNA | Identification of a large panel of B-cell epitopes | [57] |
| Human Hepatis C Virus (HCV) | Antigen discovery | Whole viral cDNA | Molecular dissection of the B-cell response | [29] |