Table 1.

The effect of different nitrogen sources on cell growth (DCW) and recombinant DPEase activity in shake flasks

Nitrogen source	Concentration (g/L)	DCW (g/L)	DPEase activity (U/mL)
Yeast extract	15	4.1 ± 0.1	137.6 ± 2.8
	25	5.2 ± 0.2	162.0 ± 3.4
	35	5.3 ± 0.1	127.7 ± 3.2
Peptone	15	4.1 ± 0.1	164.1 ± 3.5
	25	5.1 ± 0.1	189.9 ± 2.8
	35	5.4 ± 0.2	186.4 ± 2.6
Yeast decoction	15	3.2 ± 0.1	122.4 ± 3.9
	25	3.4 ± 0.1	128.4 ± 2.9
	35	4.0 ± 0.1	142.6 ± 3.5
Corn steep powder	5	2.6 ± 0.1	92.4 ± 1.9
	15	4.3 ± 0.1	167.0 ± 3.3
	25	4.4 ± 0.1	111.1 ± 2.1
	35	5.7 ± 0.2	109.0 ± 2.9
Cottonseed meal	15	3.2 ± 0.1	123.8 ± 2.7
	25	4.7 ± 0.2	199.4 ± 3.9
	35	6.2 ± 0.2	213.7 ± 4.8