Fig. 4.

LINC00511 targeted the miR-185-3p/E2F1 in the breast cancer cells as competing endogenous RNA. a The subcellular position of LINC00511 on the cytoplasm or nucleus. GAPDH and U1 acted as the cytoplasm and nuclear control. b The binding sites of miR-185-3p and LINC00511. c miR-185-3p expression in the breast cancer cells MDA-MB-231, and MCF-7 measured by RT-PCR. d miR-185-3p expression in the transfection of LINC00511 silencing and enhanced LINC00511 plasmid. e miR-185-3p and E2F1 3’-UTR binding sites. f E2F1 mRNA expression in the breast cancer cells MDA-MB-231, and MCF-7 measured by RT-PCR. g E2F1 mRNA expression in the miR-185-3p inhibitor (miR-185-3p inhib) transfection and enhanced LINC00511 plasmid transfection. ** indicates p value less than 0.01. * indicates p value less than 0.05